Figure 2: XMJ reduces oxidative stress in HUVECs treated with H2O2.

HUVECs were pretreated with XMJ (25, 50, 100 μg/ml) for 30 minutes and then incubated with H2O2 (0.2 mM) for 2 hours. (A) Distributions of eNOS dimer and monomer were determined by cold western blot analysis. (B) ROS productions were detected by DHE fluorescence. (C) MDA levels in total cell lysates. (D) SOD activity in total cell lysates. All data were expressed as mean ± SEM. N is 3 in each group. *P < 0.05 VS Control, #P < 0.05 VS H2O2 alone. NS indicates no significance.