Figure 2: Time-series profiles of intercellular metabolites. | Scientific Reports

Figure 2: Time-series profiles of intercellular metabolites.

From: A Systematic Approach to Time-series Metabolite Profiling and RNA-seq Analysis of Chinese Hamster Ovary Cell Culture

Figure 2

Intracellular metabolites measured by LC-MS/MS were classified by visual inspection (A–D) Unclassified metabolites are listed in one group (E) Metabolite concentrations are indicated on the vertical axis (μM), and time on the horizontal axis. Diamonds, squares, and triangles indicate data from control, 5 mM, and 10 mM lactate-containing cultures, respectively (triplicate experiments). Abbreviations: 6-phospho-D-gluconate (6PG), 3-phospho-D-glycerate (3PG), Iso-Citrate (Iso-Cit), Phosphoenolpyruvate (PEP), Glyceraldehyde 3-phosphate (GAP), Acetyl-CoA (AcCoA), Sedoheptulose 7-phosphate (S7P), Oxaloacetate (OXA), 2-oxoglutarate (AKG), D-Fructose 1,6-bisphosphate (D-F16P), D-Fructose 6-phosphate (D-F6P), D-Ribose 5-phosphate (D-R5P), D-Glucose 6-phosphate (D-G6P), D-Xylulose 5-phosphate (D-X5P), D-Ribulose 5-phosphate (D-Ru5P), D-Erythrose 4-phosphate (D-E4P), pyruvate (PYR), dihydroxyacetone phosphate (DHAP), L-Serine (L-Ser), L-Asparagine (L-Asn), L-Leucine (L-Leu), L-Tryptophan (L-Try), L-Phenylalanine (L-Phe), L-Cysteine (L-Cys), D-Glucose 1-phosphate (D-G1P), L-Tyrosine (L-Tyr), L-Arginine (L-Arg), L-Aspartic acid (L-Asp), L-Glutamate (L-Glu), L-Proline (L-Pro), L-Glycine (L-Gly), L-Alanine (L-Ala), L-Citrulline (L-Cit), L-Lysine (L-Lys).

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