Figure 1: Tet expression and 5hmC levels are increased during myoblast differentiation. | Scientific Reports

Figure 1: Tet expression and 5hmC levels are increased during myoblast differentiation.

From: Ten-Eleven Translocation-2 (Tet2) Is Involved in Myogenic Differentiation of Skeletal Myoblast Cells in Vitro

Figure 1

(A) Left: immunofluorescence of 5hmC (top row) or 5mC (bottom row) in C2C12 cells after differentiation induction for 6 d. Differentiated myotubes were marked by immunostaining for myosin heavy chain (MyHC) and nuclei were stained with DAPI. Scale bar, 100 μm; Right: quantification of 5hmC and 5mC immunofluorescence intensities in nuclei of differentiated and undifferentiated cells. Data are presented as means ± SEM. *p < 0.05; ***p < 0.001. (B) qRT-PCR analysis for the expression of Tet family genes in C2C12 cells at differentiation day 0, 2, 4 and 6. Gapdh was used as an internal control. Data are presented as means ± SEM (n = 3). Asterisks above columns represent significant difference among groups (p < 0.05). (C) Western blot analysis of Tet2 protein in C2C12 cells at differentiation day 0 and 6. MyHC is a marker of muscle differentiation and β-actin was used as an internal control. The expected Tet2 band (NW: 224 kDa) is shown. Full-length blots are presented in Supplementary Figure S6.

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