Figure 2: Knockdown of Tet2 decreases the expression of myoblast differentiation-associated genes. | Scientific Reports

Figure 2: Knockdown of Tet2 decreases the expression of myoblast differentiation-associated genes.

From: Ten-Eleven Translocation-2 (Tet2) Is Involved in Myogenic Differentiation of Skeletal Myoblast Cells in Vitro

Figure 2

(A) and (B) Knockdown of Tet expression. C2C12 cells were transfected with Tet1 or Tet2 siRNAs (siTet) or control siRNA (siCtrl). Forty-eight hours after transfection, mRNA levels of Tet1, Tet2 and muscle-specific genes were analyzed by qRT-PCR. Tet1 or Tet2 mRNA levels were decreased by more than 50% by their specific siRNAs when compared to that by control siRNA (left row in A or B). The expression of several muscle-specific genes was downregulated in Tet1 or Tet2 knockdown cells (right row in A or B). Note that Tet2 knockdown led to dramatic decrease of myogenin (Myog), Myf6 and myomaker (MyoM) expression. *p < 0.05; **p < 0.01; ***p < 0.001 as compared with C2C12 transfected by control siRNA. (C) The expression levels of muscle-specific genes during C2C12 differentiation. Gapdh was used as an internal control. Data are presented as means ± SEM (n = 3). Asterisks above columns represent significant difference among the groups (p < 0.05).

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