Figure 7: Proposed model of Tet2-involved myoblast differentiation.

Tet2 expression is upregulated in myoblasts after differentiation induction. Tet2 directly induces hydroxymethylation and subsequent demethylation of differentiation-associated genes (eg, myogenin and myomaker). Besides, downregulation of Dnmt1 may lead to a passive demethylation in this process. Demethylation results in activation and expression of the differentiation-associated genes, which drives myotube formation.