Table 3 PaLoc of isolates 103, 106 and 133 compared with other Clade 3 strains and a representative strain of each Clade 1, 2 and 5.

From: Whole genome sequences of three Clade 3 Clostridium difficile strains carrying binary toxin genes in China

Clade

Strain

tcdR

tcdB

tcdE

Tn6218

tcdA

tcdC

int

xis

rep

xre

merR

oxidoreductase

flavodoxin

orf

σ70

1

CD630

12 (6NS)

92 (48NS)

5 + 31nt del + 1nt in (7NS + 10 del)

116 (44NS)

5 + 318nt del (1NS + 106 del)

2

CD196

12 (6NS)

446 (175NS)

3 + 31nt del + 1nt in (6NS + 10 del)

89 + 63nt del (34NS + 21 del)

4 + 36nt del (12 del)

5

M120

18 (8NS)

173 (87NS)

4 + 42nt in (2NS + 14 in)

89SNP + 63nt del (44NS + 21 del)

3 + 15nt del (3NS + 5 del)a

3

ZJCDC-S82

ID

6 (3NS)

ID

ID

ID

5 (3NS)

ID

ID

ID

ID

ID

11 + 210nt in (5NS + 70 del)

18 (11NS)

36nt del (12 del)

CD69

ID

12 (7NS)

ID

3 (4NS)

ID

5 (3NS)

ID

ID

ID

ID

ID

ID

153 (73NS)

ID

VL-0391

ID

2 (2NS)

ID

7 (4NS)

ID

3 (3NS)

1 (1NS)

ID

1 (1NS)

1 (1NS)

ID

1 (0NS)

11 + 36nt del (5NS + 12 del)

36nt del (12 del)

VL-0104

ID

2 (2NS)

ID

8 (4NS)

ID

3 (3NS)

1 (1NS)

ID

1 (1NS)

1 (1NS)

ID

1 (0NS)

11 + 36nt del (5NS + 12 del)

36nt del (12 del)

L058

1 (1NS)

5 (2NS)

ID

5 (3NS)

ID

3 (2NS)

ID

ID

2 (2NS)

ID

ID

ID

8 (4NS)

ID

OX561

1 (1NS)

6 (3NS)

ID

9 (3NS)

1 (1NS)

2 (2NS)

ID

ID

1 (1NS)

ID

ID

ID

7 + 63nt del (5NS + 21 del)

ID

OX1232

1 (1NS)

6 (3NS)

ID

8 (3NS)

ID

2 (2NS)

ID

ID

1 (1NS)

ID

ID

ID

14 (10NS)

ID

OX1485

1 (1NS)

5 (3NS)

ID

8 (3NS)

ID

2 (2NS)

ID

ID

1 (1NS)

ID

ID

ID

37 (21NS)

ID

OX2183

ID

3 (3NS)

ID

ID

47nt in (9 in)

5 (3NS)

ID

ID

ID

ID

ID

ID

51 (23NS)

36nt del (12 del)

Q24

ID

8 (4NS)

ID

7 (2NS)

1 (1NS)

2 + 108nt in (1NS + 36 in)

2 (1NS)

1 (1NS)

1 (1NS)

3 (0NS)

2 (0NS)

ID

62 (35NS)

36nt del (12 del)

  1. The results shown in the Table are that the PaLoc sequence of isolate 103, 106 and 133 queries those of strains listed in the Strain column. The number of SNP is shown in the column with the resulted amino acid substitutions and deletions being indicated in parentheses. ID, identical; in, insertion; del, deletion; -, absent; nt, nucleotide; NS, non-synonymous SNP. The nucleotide deletions and insertions are consecutive. atcdC of strain NAP08 has a nonsense mutation, resulting in 37 aa deletions of the C-terminal end compared to those of isolates 103, 106 and 133.