Figure 4: Sequence analysis design. The bar stands for gene sequences.
From: Genetic and Proteomic characterization of Bile Salt Export Pump (BSEP) in Snake Liver

The first sequence was obtained through PCR amplification from a conserved region of predicted sequences from different snakes (â‘ ), extended sequences were subsequently obtained with primers based the first sequences and the other parts of predicted sequences (â‘¡), finally, more snakes liver samples was used to re-test the existence of BSEP.