Figure 3: Differential glycomic profiling in kidney.

(a) Hematoxylin-stained kidney section image annotated with the inner and outer cortex, medulla, and pelvic regions. (b) PCA of the normalized lectin microarray data on the tissue fragments from kidney of Mouse 2 (total 12 samples). Each point represents one tissue fragment. (c) Two-dimensional HC analysis of the lectin microarray data shown in (b). The 12 samples are listed in columns and the 45 lectins are listed in rows. The color and intensity of each square indicate the lectin signal levels in specific tissue fragments (Red, high; green, low; black, medium). (d) Histochemical validation of differential glycome profiling in kidney by double staining with AAL recognizing fucose (green) and SNA recognizing α2,6-linked sialic acid (red) (A), and by staining with LEL recognizing GlcNAc oligomer (green) (B). The enlarged images of three positions in cortex (i), cortex-medulla interface (ii), and medulla (iii) are shown.