Figure 4: Histochemical validation of differential glycomic profiling in testis by lectin staining.
From: A standardized method for lectin microarray-based tissue glycome mapping
The results of the lectin microarray were histochemically confirmed by lectin staining with (a) HPA recognizing α-linked terminal GalNAc residue (Tn-antigen), (b) PNA recognizing the Galβ1-3GalNAc structure (T-antigen), and (c) WFA recognizing both the terminal GalNAc residue and Galβ1-3GalNAc structure. (d) GNA recognizing high-mannose type N-glycan was used as a control. (e) PBS was used as the negative control (N.C.) instead of these lectins.
