Figure 4: Representative images of immunohistochemical staining of PRX3, periodic acid-Schiff and Masson’s trichrome staining of kidney tissues from patients with acute tubular necrosis (ATN) and normal controls.

(A) Asterisks indicate the blood vessels. (B) Tubular injury score, (C) inflammatory cell infiltration, and (D) the percentage of interstitial fibrosis were assessed by computer-assisted quantitative methods. Patients were stratified into high and low expression groups by the cutoff value of 0.2492 for tubular PRX3 QISV based on the ROC curve analysis. Data are expressed as mean ± SD. #P < 0.05; ##P < 0.01. Scale bars, 50 μm. PRX3, peroxiredoxin 3; QISV, quantitative immunohistochemical staining value; ROC, receiver operating characteristic; SD, standard deviation.