Figure 1: IL-1β suppresses stem cell self-renewal gene expression in mouse NSCs. | Scientific Reports

Figure 1: IL-1β suppresses stem cell self-renewal gene expression in mouse NSCs.

From: Inflammation-induced miRNA-155 inhibits self-renewal of neural stem cells via suppression of CCAAT/enhancer binding protein β (C/EBPβ) expression

Figure 1

(A) Morphological changes of NSCs treated with 1 ng/ml IL-1β. (B) Difference in cell proliferation between mock control (PBS, Cont) and 1 ng/ml IL-1β-treated cells. Cell numbers were normalized by initial cell number (1 × 105 cells/ml). The asterisks represent a significant difference (P < 0.05) between the groups. (C) qPCR for genes related to NSC self-renewal. Specimens were collected at each time point after 1 ng/ml IL-1β administration. Characters a–c represent significant differences among groups (P < 0.05) determined by Tukey-Kramer HSD test for multiple comparison. Briefly, the different characters above each column (e.g. a and b) represent significant differences among the columns and the same characters above different columns (e.g. a and a) show no significant difference. (D) Western blots for Nestin, Nf-M, Msi1, Hes1 and Bmi1 for NSCs treated with 1 ng/ml IL-1β administration. (E), Western blot for Caspase 3, demonstrating no measurable change cleaved-caspase related to apoptosis.

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