Figure 2: miR-155 is involved in IL-1β-induced suppression of self-renewal genes. | Scientific Reports

Figure 2: miR-155 is involved in IL-1β-induced suppression of self-renewal genes.

From: Inflammation-induced miRNA-155 inhibits self-renewal of neural stem cells via suppression of CCAAT/enhancer binding protein β (C/EBPβ) expression

Figure 2

(A) IL-1β-induced expression of mmu-miR-155 (miR-155). The y-axis represents expression relative to the no-treatment control (NTC). U6 small nuclear RNA (snRNA) was used as an internal control. The asterisks represent a significant difference (P < 0.05) between the groups. (B) qPCR for mature miR-155, Msi1, Hes1 and Bmi1 in NSCs treated with the scrambled oligonucleotide (SCR, control), the miRNA inhibitor oligonucleotide to mmu-miR-155 (inhibitor) and 1 ng/ml IL-1β. Treatment with the inhibitor ameliorated suppression of Msi1, Hes1 and Bmi1 expression by IL-1β. Characters a-c represent significant differences among groups (P < 0.05) determined by Tukey-Kramer HSD test for multiple comparison. (C) Western blots for Msi1, Hes1 and Bmi1 for NSCs treated with the SCR, inhibitor and IL-1β.

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