Figure 3: Over-expression of miR-155 leads to suppression of the self-renewal genes Msi1, Hes1 and Bmi1 and inhibition of self-renewal. | Scientific Reports

Figure 3: Over-expression of miR-155 leads to suppression of the self-renewal genes Msi1, Hes1 and Bmi1 and inhibition of self-renewal.

From: Inflammation-induced miRNA-155 inhibits self-renewal of neural stem cells via suppression of CCAAT/enhancer binding protein β (C/EBPβ) expression

Figure 3

(A) qPCR for Msi1, Hes1 and Bmi1 for NSCs transfected with the GFP-NTC (control) and the GFP-mmu-miR-155 (miR-155) plasmids. Asterisks represent significant differences (P < 0.05) compared with control. (B) Western blots for Msi1, Hes1 and Bmi1 for NSCs transfected with the control and miR-155 plasmids. (C) Western blot for caspase 3 for NSCs transfected with control and miR-155 plasmids. (D) Cumate induction of miR-155 and GFP expression in NSCs transfected with pPBQM-miR155-IRES-GFP. (E) qPCR for Msi1, Hes1 and Bmi1 for NSCs stably expressing pPBQM-miR155-IRES-GFP (QM-miR155). Asterisks represent significant differences (P < 0.05) among groups. (F) Rates of cell proliferation for NSCs stably expressing QM-miR155 with and without cumate treatment. Cell numbers were normalized by cell numbers for the control group at 3 days of culture. The asterisk represents a significant difference (P < 0.05).

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