Figure 4: C/EBPβ is involved in miR-155-mediated attenuation of the stem cell self-renewal genes in NSCs. | Scientific Reports

Figure 4: C/EBPβ is involved in miR-155-mediated attenuation of the stem cell self-renewal genes in NSCs.

From: Inflammation-induced miRNA-155 inhibits self-renewal of neural stem cells via suppression of CCAAT/enhancer binding protein β (C/EBPβ) expression

Figure 4

(A) Quantitative RT-PCR for C/EBP family members in NSCs overexpressing miR-155. The control was NSCs transfected with a scrambled sequence. The asterisk represents a significant difference (P < 0.05) compared with the control. (B) Western blots for C/EBPα and β after overexpression of miR-155. The control was NSCs transfected with a scrambled sequence. (C) Suppression of C/EBPβ expression, as well as levels of Msi1, Hes1 and Bmi1. NTC = no-treatment control. SCR = scrambled oligonucleotide sequence RNA-transfected control. Asterisks represent significant differences (P < 0.05) compared with the NTC and SCR groups. (D) Western blots for Msi1, Hes1 and Bmi1 for NSCs treated with C/EBPβ siRNA. (E) Binding motifs for C/EBP transcription factors in the target regions used for ChIP-PCR analysis. (F) ChIP-PCR analysis for DNA immunoprecipitated by anti-C/EBPβ antibody.

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