Figure 5: Suppression of target genes by miR-155 is conserved in human NSCs derived from human iPS cells. | Scientific Reports

Figure 5: Suppression of target genes by miR-155 is conserved in human NSCs derived from human iPS cells.

From: Inflammation-induced miRNA-155 inhibits self-renewal of neural stem cells via suppression of CCAAT/enhancer binding protein β (C/EBPβ) expression

Figure 5

(A) NSCs induced from human iPS cells (a), and NESTIN and NF-M expressing neural cells derived from NSCs (b). (B) quantitative RT-PCR for C/EBPβ, MSI1, HES1 and BMI1 for NSCs derived from human iPS cells. The NSCs were treated with hsa-miR-155 mimic oligonucleotide (Mimic) or inhibitor to hsa-miR-155 (Inhibitor). The control was NSCs transfected with a scrambled sequence. Asterisks represent significant differences (P < 0.05) compared with the control. (C) Western blots for miR-155 targets for NSCs treated with the mimic oligonucleotide. (D) Western blots for miR-155 targets for NSCs treated with the inhibitor oligonucleotide.

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