Figure 1: Pin1 knockdown suppresses the proliferation of human HCC cells in vitro. | Scientific Reports

Figure 1: Pin1 knockdown suppresses the proliferation of human HCC cells in vitro.

From: Chemical or genetic Pin1 inhibition exerts potent anticancer activity against hepatocellular carcinoma by blocking multiple cancer-driving pathways

Figure 1

PLC/PRF/5 (A) or Huh-7 (C) cell lines were infected with lentiviruses expressing scrambled or Pin1 shRNA. Cell lysates were subject to western blot analysis with antibodies against Pin1 or internal control Actin. Growth curves of PLC/PRF/5 (B) and Huh-7 (D) cell lines with Pin1 knockdown were plotted over time, based on the cell numbers counted daily. 3 × 104 cells for both cell lines were placed in each well of 12-well plate with triplicate for each time point. Error bars represent standard deviations (N = 3). p values were derived from the cell numbers at the end point. (E,F) Morphological change of PLC/PRF/5 (E) and Huh-7 (F) cells after Pin1 knockdown. (G,H) Pin1 knockdown affects cells migration in PLC/PRF/5 (G) and Huh-7 (H), as evaluated by transwell assay. 5 × 104 cells for both cell lines were used for this assay. Error bars represent standard deviations (n = 2).

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