Figure 4: Transcriptomic analysis of CIH-derived aorta macrophages.

(A) Volcano plot showing differentially expressed transcripts between CIH-derived and RA-derived aorta macrophages (n = 8 per group). We identified 16,343 differentially expressed transcripts between the groups (p < 0.01, blue points). X axis depicts the extent of the difference (log2 Fold Changes) between the groups, whereas Y axis depict the significance of this difference (log10(adjusted p-value)). (B) Unsupervised hierarchical clustering of differentially expressed transcripts separate the samples between the two experimental groups. Expression levels are represented as a color gradient in the heatmap, from red (upregulated in CIH) over white (no changes) to blur (upregulated in RA). (C) Overrepresented canonical pathways in genes differentially regulated between CIH and RA in aorta macrophages. Blue bars represent the significance of the overrepresentation (−log(p-value)). Orange line depicts the ratio between the number of genes in each canonical pathway and the number of genes differentially expressed in CIH-derived compared to RA-derived aorta macrophages.