Figure 1: Aggregation of neuroserpin with statins or botulin.
From: Cholesterol impairment contributes to neuroserpin aggregation
(a) 100 μg protein of HeLa cells or treated with 10 μM PRA or 5 μM SIM or 0.1 μM rosuvastatin or 1 μM rosuvastatin or 20 μM betulin were loaded on 10% native gel and transferred on PVDF membrane. The sheet was incubated with anti-neuroserpin (ab32901, Abcam, 1:500) overnight at 4 °C and then with the secondary antibody anti goat-HRP (1:5000, ECL Blotting reagents (GE Healthcare RPN2109)/SuperSignal West Femto Maximum Sensitivity Substrate, Thermo scientific) for 1 h at room temperature. Ponceau S solution (P7170, Sigma) was used to verify the correct loading of the samples. (b) Effect of statins or betulin treatments on the level of expression of neuroserpin or HMGCR. 10 μg total protein were loaded on 10% polyacrilamide gel, transferred on PVDF and incubated with anti-neuroserpin (ab32901, AbCAM, 1:500) or with anti-HMGCR (1:1000, MAB5374, Millipore) overnight at 4 °C). Vinculin (1:10000, Sigma) was used as housekeeping.
