Figure 3: Model summarizing the core PhoP regulon in P. luminescens. | Scientific Reports

Figure 3: Model summarizing the core PhoP regulon in P. luminescens.

From: An antimicrobial peptide-resistant minor subpopulation of Photorhabdus luminescens is responsible for virulence

Figure 3

(A) Two sets of PhoP-dependent genes (55 LB and 64 low Mg2+) were identified after RNAseq analysis of the gene expression patterns of the TT01 and phoP strains in two different sets of growth conditions (LB medium or minimal M9 medium supplemented with 10 μM Mg2+). The intersection of the two lists identifies 21 gene markers as the core PhoP regulon (see box). Bold thin arrows indicate direct regulation by the phosphorylated form of PhoP (P-PhoP). Hatched thin arrows indicated that the binding profile is unknown. CHP: conserved hypothetical protein, T6SS: type 6 secretion system. (B) Electrophoretic mobility shift assays were carried out to test the binding of the PhoP protein activated in vitro with 10 mM acetyl phosphate (P-PhoP) or of the non-activated PhoP-His to the 206-bp phoP and 198-bp pbgPE promoter regions. The PhoP-His concentrations indicated are in micromoles per liter. We checked that binding was specific, by adding BSA and poly(dI-dC) to the binding buffer to saturate non-specific binding sites.

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