Figure 4: Mass spectrometry analysis of the rLpqHHis microheterogeneity. | Scientific Reports

Figure 4: Mass spectrometry analysis of the rLpqHHis microheterogeneity.

From: Scrutiny of Mycobacterium tuberculosis 19 kDa antigen proteoforms provides new insights in the lipoglycoprotein biogenesis paradigm

Figure 4

(A) Fragmentation pattern of the hepta glycosyl triacylated rLpqHHis proteoform [Hex7,GroC16C16C19] obtained by top-down CID (Gro: Glycerol; R1 + R2 + R3 = C51; Precursor ion: 1810.029+). (B) Chromatographic separation of the different glycoforms (from 0 to 9 hexoses) of the tryptic peptide [S27-K51]. (C) Localization of the first 4 sequential glycosylation sites on rLpqHHis obtained from bottom-up analysis of the [S27-K51] glycoforms.

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