Figure 3: Increased IRF-1 facilitated P38 phosphorylation and aggravated liver injury, whereas SB203580 inhibited P38 activation and reduced liver injury. | Scientific Reports

Figure 3: Increased IRF-1 facilitated P38 phosphorylation and aggravated liver injury, whereas SB203580 inhibited P38 activation and reduced liver injury.

From: Interferon regulatory factor-1 activates autophagy to aggravate hepatic ischemia-reperfusion injury via the P38/P62 pathway in mice

Figure 3

(a) Western blot analysis of IRF-1, P38, p-P38 and caspase-3 levels in livers from 12 h-IR mice pretreated with AdGFP (empty adenoviral vector control) or AdIRF-1 (adenoviruses encoding murine IRF-1). (b) AdIRF-1 pretreatment increased ALT levels, which were decreased by SB203580. (c) Representative histopathological images of livers as described in (a). H&E staining. (d) Western blot analysis of P38 and p-P38 levels in livers from control and IR (2, 6, 12, 24 h after reperfusion) mice. (e) Apoptosis was assessed by TUNEL assay in livers as described in (a). (f) Representative histopathological images of livers exposed to AdIRF-1 or AdIRF-1 plus SB203580. H&E staining. (g) Immunohistochemical staining of proliferating cell nuclear antigen (PCNA) in livers as described in (f). (h) Western blot analysis of P38 and p-P38 in livers as described in (f). **P < 0.01 and ***P < 0.001.

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