Figure 6: Overexpression of McMYB12a and McMYB12b in tobacco elevates different flavonoids production. | Scientific Reports

Figure 6: Overexpression of McMYB12a and McMYB12b in tobacco elevates different flavonoids production.

From: McMYB12 Transcription Factors Co-regulate Proanthocyanidin and Anthocyanin Biosynthesis in Malus Crabapple

Figure 6

(A) The phenotype of transgenic tobacco flowers over-expressing McMYB12a and McMYB12b, respectively. Flowers of pBI121-McMYB12a plants showed an increased pigmentation in petal compared to pBI121-McMYB12b and control flowers. And Flowers of pBI121-McMYB12b plants showed lighter pink than the control flowers. (B) The flavonoids compound concentrations in different samples. (C) qRT-PCR analysis of McMYB12a and b and anthocyanin pathway genes in the petals each of three different transformed 35 S::McMYB12s tobacco lines. pBI121-McMYB12a 1#, pBI121-McMYB12a 3# and pBI121-McMYB12a 5#, three independent McMYB12a-overexpressing lines; pBI121-McMYB12b 2#, pBI121-McMYB12b 7# and pBI121-McMYB12b 9#, three independent McMYB12b-overexpressing lines. Error bars indicate the mean ± SE of three replicate reactions. Different letters above the bars indicate significantly different values (P < 0.05) calculated using one-way analysis of variance (ANOVA) followed by a Tukey’s multiple range test.

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