Figure 1: Immunocytochemical evaluation of postnatal OTCs at DIV 7. | Scientific Reports

Figure 1: Immunocytochemical evaluation of postnatal OTCs at DIV 7.

From: Time-lapse imaging reveals highly dynamic structural maturation of postnatally born dentate granule cells in organotypic entorhino-hippocampal slice cultures

Figure 1

(a) Resliced OTC sections (50 μm) prepared on P 4-5 and fixed on DIV 7 were processed for immunoreactivity of the immature neuronal marker doublecortin (DCX; green), the mature neuronal marker calbindin (CB; red), and the granule cell marker Prox1 (blue). (b) Expression of DCX was observed in the subgranular zone (SGZ), the inner and middle parts of the granule cell layer (GCL) in cell somata, as well as dendrites that extended into the molecular layer (ML). Calbindin labeling was detected in dentate granule cells (GCs) that were located in the outer parts of the GCL with processes that reached the hippocampal fissure (fis). Prox1 was expressed in both, immature and more mature GCs in the GCL. DCX and calbindin co-localized only rarely, while Prox1 was co-expressed with both of these markers in addition to being present in other GCs as well. Scale bars: (a) 50 μm; (b) 10 μm.

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