Figure 3: Retroviral (RV) transduction of newborn dentate granule cells (GCs) by local injection. | Scientific Reports

Figure 3: Retroviral (RV) transduction of newborn dentate granule cells (GCs) by local injection.

From: Time-lapse imaging reveals highly dynamic structural maturation of postnatally born dentate granule cells in organotypic entorhino-hippocampal slice cultures

Figure 3

(a) A triple immunostaining of an OTC that was transduced with RV-GFP on DIV 0 and fixed on 14 dpi. DCX immunoreactivity was observed in the subgranular zone (SGZ) and the inner part of the granule cell layer (GCL), while Prox1 was expressed throughout the GCL. Several RV-GFP-labeled cells located in the SGZ and inner GCL were polarized with processes extending into the GCL and the molecular layer (ML), and co-expressed Prox1 and DCX (white arrows), suggesting that a number of newborn GCs were transduced with the RV. In addition, there were RV-GFP-positive cells with glial morphology that did not express DCX, nor Prox1, (examples are denoted by asterisks). (b) A magnification shows RV-GFP-labeled cells located in the SGZ and the inner layers of the GCL co-expressing both Prox1 and DCX (white arrows). Scale bars: (a) 50 μm; (b) 10 μm.

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