Figure 5: Time-lapse imaging and reconstruction of RV-CAG-GFP-labeled postnatally born GCs and older AAV-Synapsin1-tdTomato-labeled GCs. | Scientific Reports

Figure 5: Time-lapse imaging and reconstruction of RV-CAG-GFP-labeled postnatally born GCs and older AAV-Synapsin1-tdTomato-labeled GCs.

From: Time-lapse imaging reveals highly dynamic structural maturation of postnatally born dentate granule cells in organotypic entorhino-hippocampal slice cultures

Figure 5

(a) OTCs were transduced with an RV-CAG-GFP and an AAV-Synapsin1-tdTomato vector to label newborn (RV-GFP; green) and older GCs (AAV-Syn; red) respectively. (b,c) Daily time-lapse imaging of individual RV-GFP-labeled GCs was performed over a period of three weeks between 8 and 28 days post virus injection (dpi). All cells were 3-D computer reconstructed to allow for detailed morphological analysis over time. (d,e) Example of an AAV-Syn-labeled cell with 3-D reconstructions at different time points. Scale bars: (a1) 20 μm, (a2-e) 10 μm.

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