Figure 6: Localization and expression of APP in human lean and obese adipose tissue.
From: Amyloid precursor protein modulates macrophage phenotype and diet-dependent weight gain
Human lean and obese omental (a,e) and subcutaneous (b,f) adipose tissues were sectioned and immunostained using anti-APP, CD68, Aβ (4G8, and BAM-10) antibodies and antibody binding visualized using Vector VIP as the chromogen or Alexa fluor-conjugated secondary antibodies for double labeling. Omental (c) and subcutaneous (d) adipose tissues were also lysed, resolved by 10% SDS-PAGE and western blotted using anti-APP and GAPDH (loading control) antibodies. Arrowheads indicate bands of interest when nonspecific bands are present. Antibody binding was visualized by chemiluminescence. Optical densities of the western blotted proteins were normalized against their respective GAPDH loading controls. Lean and obese omental and subcutaneous tissue was lysed to quantify soluble Aβ 1–40 and Aβ1-42 (g), TNFα (h), IL-6 (i), MDC (j), IL-8 (k), and MCP-1 (l) levels via ELISA. ELISA values were normalized to tissue weight. Data are expressed as mean +/− SD (n = 5) *p < 0.05.
