Table 1 Observed KD (KDobs) values of L-tryptophan binding to HSA (reported KD ~200 μM) measured by waterLOGSY titration at varying mixing times and protein concentrations.

From: Protein-ligand binding affinity determination by the waterLOGSY method: An optimised approach considering ligand rebinding

Mixing time/s

KDobs/μM

25 μM HSA

50 μM HSA

100 μM HSA

0.15

−

−

765 ± 140

0.25

−

−

1000 ± 200

0.5

185 ± 50

475 ± 165

1500 ± 400

0.75

200 ± 45

715 ± 230

1800 ± 100

1

280 ± 40

845 ± 270

2300 ± 200

2

510 ± 80

1700 ± 500

6500 ± 1600

  1. Errors show are standard errors from three separate experiments.
Back to article page