Figure 4: DEN induces profound changes in gene expression profile.

(a) Principal component analysis of gene signatures of each mouse. (b) Volcano plot illustrated the fold change and statistical significance of genes in DEN group compared with the control group. (c) Heatmap showed the expression profiles of control and DEN mice. (d) Top ten pathways affected by DEN from KEGG enrichment analysis, black bars represented the genes up-regulated by DEN, grey bars represented the genes down-regulated by DEN compared with control.