Table 3 Enriched canonical pathways by 18/1 patterned stimulation for 2 hr and 5 hr.

From: Gene networks activated by specific patterns of action potentials in dorsal root ganglia neurons

Pathway

# mol

Z-score

p

Molecules

2 hr

IGF-1 Signalling

7

−1.13

4.41e-3

FOS, CSNK2A2, SOCS3, SHC1, PRKAG2, STAT3, SOCS5

ERK/MAPK Signalling

8

−2.12

3.76e-2

FOS, SHC1, ITGA3, PRKAG2, DUSP4, PAK7, RPS6KA1, STAT3

Tec Kinase Signalling

7

−1.63

4.62e-2

FOS, ITGA3, GNB2, STAT2, PAK7, STAT3, ITK

Axonal Guidance Signalling

15

—

3.80e-2

RGS3, SLIT1, BDNF, NFATC3, C9orf3, EPHA1, WNT9A, PLCH2, LIMK1, SHC1, ITGA3, PRKAG2, GNB2, PAK7, VASP

5 hr

CDK5 Signalling

20

−0.47

1.07e-2

ITGB1, PPP1R14C, RAF1, BDNF, PPP2R5D, PPP2R5B, ADCY6, MAPK12, CACNA1A, CDK5R1, ADCY9, PPP1R3D, ITGA3, PPP1R12A, MAP2K2, CAPN1, PRKAG2, MRAS, PPP2R5E, MAPK7

Death Receptor Signalling

19

2.07

9.45e-3

MAP2K4, GAS2, RELA, MAP2K7, PARP8, TNKS2, FAS, ACIN1, DAXX, TRADD, TRAF2, CASP9, CRADD, TNFSF12, BID, HTRA2, CHUK, NFKBIB, TNFRSF10A

Rac signalling

20

1.34

2.29e-2

MAP2K4, ITGB1, RELA, RPS6KB1, RAF1, MAP2K7, MAP3K11, ARPC5L, MAP3K1, PIKFYVE, PIP4K2B, WASF1, CDK5R1, PAK1, ITGA3, MAP2K2, BAIAP2, MRAS, PIP5KL1, PAK7

ERK5 signalling

13

1.73

3.76e-2

IL6ST, RPS6KB1, BAD, CREB3, CREBBP, MYC, RPS6KA6, MEF2D, NTRK1, MRAS, FOSL1, MAPK7, RPS6KA1

NGF signalling

19

1.41

4.20e.2

MAP2K4, GAS2, RELA, MAP2K7, PARP8, TNKS2, FAS, ACIN1, DAXX, TRADD, TRAF2, CASP9, CRADD, TNFSF12, BID, HTRA2, CHUK, NFKBIB, TNFRSF10A

  1. IPA analysis was performed on up- and down-regulated transcripts (>|1.4|-fold and p < 0.05) as described in Fig. 2 and mapped to cellular and molecular signalling pathways. Z-score represents the predicted pathway activation (+) or inhibition. Significance values were calculated by a Fisher’s exact test (right-tailed) to determine the probability that a pathway was attributable to chance alone. Enriched pathways are shown, demonstrating opposing activation (18/1) and inhibition (90/5) for Nerve Growth Factor (NGF) and Rac signalling (Fig. 3) important for neurite outgrowth.
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