Table 1 Primer sets for real-time PCR amplification of proinflammatory cytokines.
From: Influence of extracellular zinc on M1 microglial activation
Accession number | Primer sequences | Annealing temperature | |
|---|---|---|---|
IL-1β | NM_008361 | Forward 5′-CTCTTGTTGATGTGCTGCTG-3′ | 60 °C |
Reverse 5′-GACCTGTTCTTTGAAGTTGACG-3′ | |||
iNOS | NM_ 010927 | Forward 5′-CACTTCTGCTCCAAATCCAA-3′ | 60 °C |
Reverse 5′-GACTGAGCTGTTAGAGACACTT-3′ | |||
IL-6 | NM_031168 | Forward 5′-TCCTTAGCCACTCCTTCTGT-3′ | 60 °C |
Reverse 5′-AGCCAGAGTCCTTCAGAGA-3′ | |||
TNFα | NM_013693 | Forward 5′-TCTTTGAGATCCATGCCGTTG-3′ | 60 °C |
Reverse 5′-AGACCCTCACACTCAGATCA-3′ | |||
β-actin | NM_007393 | Forward 5′-GACTCATCGTACTCCTGCTTG-3′ | 60 °C |
Reverse 5′-GATTACTGCTCTGGCTCCTAG-3′ | |||
