Figure 1: Linear progression of metastases.
From: Genomic Analyses of Breast Cancer Progression Reveal Distinct Routes of Metastasis Emergence
Allele frequency plots and phylogenetic trees for (a) P15, (b) P46 and (c) P123. Allele frequency plots, based on targeted deep sequencing data, comparing frequencies of validated somatic mutations in the coding region between different steps of malignant progression. Each dot represents a somatic non-synonymous, splicing or synonymous somatic mutation. The color code indicates the step at which the mutation appears for the first time. Dots in blue, red, green and purple depict mutations appearing for the first time in pre-invasive tissue, primary tumor, synchronous axillary lymph node metastases (sALN) and asynchronous distant metastasis (aDM), respectively. The arrows indicate the level corresponding to the diploid, heterozygous allele frequency of the sample, derived from Bubble Tree analysis. Phylogenetic trees depict the clonal evolution through cancer progression. An increase in color intensity reflects the acquisition of additional somatic mutations.
