Figure 1: Hierarchical clustering of INO80 complexes in wild type and genetic deletion backgrounds.
From: Identification of Topological Network Modules in Perturbed Protein Interaction Networks
(A) A total of 14 different subunits of the Ino80 complex were used as baits for the TAP purification and then analyzed by MudPIT and dNSAF label-free quantitation. Hierarchical clustering of the 31 total analyses is shown with dNSAF values as input. Proteins that are yeast specific are in red, while proteins that are orthologous to human proteins are in black. (B) The INO80 complexes from seven different deletion strains were purified using Ino80 as the TAP-tagged subunit and analyzed by MudPIT. Four biological replicates of wild type INO80-TAP are clustered with four biological replicates of INO80-TAP IES4Δ, four biological replicates of INO80-TAP ARP8Δ, four biological replicates of INO80-TAP ARP5Δ, three biological replicates of INO80-TAP IES2Δ, one analysis of INO80-TAP IES5Δ, and one analysis of INO80-TAP NHP10Δ. The proteins belonging to the ARP8, ARP5, and NHP10 modules are in blue, green and red, respectively, while proteins that were not significantly altered by the mutants are in black. In both (A) and (B), the color intensity represents protein abundance with bright yellow displaying highest abundance and black indicating that the protein was not detected in a particular purification.
