Figure 2: Normalization with various detergents.

(A) Fluorescence after addition of the same volume of buffer or detergent solution (final concentration is 0.83% wt/wt for DDM and 0.67% vol/vol for Triton) to a liposome solution with 18 mM lipids. To ensure a complete dissociation of the liposomes and the absence of FRET within micelles, detergent was added up to 55 mM for Triton (more than 200 times its CMC) and up to 80 mM for DDM (more than 500 times its CMC), without any change in the outcome. Only fluorescent lipids concentrations below 0.01% have been tested because they provide fluorescence intensities close to that of infinite dilution (see Fig. 1). Fluorescence is decreased upon addition of detergent. The values are normalized by the average fluorescence after addition of buffer (crosses). All standard deviations are within the size of the markers. (B) Relative fluorescence intensities after addition of DDM, Triton or β-OG (final concentration 0.83% wt/wt, 0.67% vol/vol and 0.83% wt/wt respectively) to a liposome solution with 1 mM lipids and containing 1.5% NBD-DOPE and 1.5% Rho-DOPE. The values are normalized by the average fluorescence produced by DDM in panel A. Error bars are standard deviations on more than 50 wells. In both panels, the temperature is set at 37 °C.