Figure 3: Subclass of PYLs Inhibits PP2Cs in the presence of (+)/(−)-iso-PhABA.
From: Design and Functional Characterization of a Novel Abscisic Acid Analog
(A) (+)/(−)-iso-PhABA mediated the inhibition of HAB1 phosphatase activity by PYLs. The concentration for each PYL protein was 5 μM and was 3.0 μM for HAB1 and 10 μM for (+)/(−)-iso-PhABA. All experiments were repeated three times (n = 3), and error bars represent the s.d. The conditions measuring the phosphatase activity of HAB1 were the same throughout, unless otherwise noted. The relative phosphatase activity of each reaction was normalized to that of the reaction with phosphopeptide substrate and HAB1 (100%). (B) PYL10 inhibits HAB1 phosphatase more strongly in the presence of (+)/(−)-iso-PhABA than (+)-ABA.
