Table 1 Determination of chiral amino acids in 9030617O03Rik (+/+) and (−/−) mouse hearts by two-dimensional high-performance liquid chromatography (2D-HPLC).
L-analogue | D-analogue | |||
|---|---|---|---|---|
+/+ | −/− | +/+ | −/− | |
His | 896.2 ± 67.1 | 920.1 ± 106.9 | nd | nd |
Asn | 899.6 ± 97.2 | 1059.9 ± 246.4 | nd | nd |
Ser | 1572.8 ± 142.8 | 1272.6 ± 380.4 | 10.2 ± 2.6 | 4.2 ± 0.8 |
Gln | 6673.3 ± 224.2 | 6934.9 ± 354.7 | nd | nd |
Arg | 1440.4 ± 78.5 | 1082.2 ± 76.7 | 4.95 ± 1.1 | nd |
Asp | 5618.5 ± 286.0 | 6216.7 ± 252.3 | 19.7 ± 0.8 | 15.2 ± 0.9 |
Gly | 2123.2 ± 26.5 | 2010.0 ± 81.8 | — | — |
allo-Thr | nd | nd | nd | nd |
Glu | 26193.9 ± 1296.6 | 25835.5 ± 1027.8 | 31.0 ± 1.3 | 187.3 ± 11.5 * |
Thr | 1523.2 ± 83.4 | 1181.8 ± 137.7 | nd | nd |
Ala | 10048.3 ± 603.1 | 10119.0 ± 630.2 | 10.2 ± 1.8 | 3.5 ± 0.4 |
Pro | 460.5 ± 44.4 | 319.8 ± 25.9 | 9.0 ± 1.3 | 5.2 ± 0.9 |
Met | 296.3 ± 25.1 | 239.9 ± 18.5 | nd | nd |
Val | 759.6 ± 81.2 | 663.6 ± 16.1 | nd | nd |
allo-Ile | nd | nd | nd | nd |
Ile | 263.3 ± 22.1 | 234.5 ± 13.3 | nd | nd |
Leu | 652.0 ± 48.3 | 529.4 ± 1.6 | 3.3 ± 1.0 | nd |
Phe | 346.0 ± 19.5 | 298.5 ± 16.6 | nd | nd |
Trp | 74.8 ± 23.9 | 44.7 ± 4.7 | nd | nd |
Lys | 1464.7 ± 119.7 | 1334.9 ± 161.6 | nd | nd |
Cys | nd | nd | nd | nd |
Tyr | 475.2 ± 58.8 | 355.4 ± 40.0 | nd | nd |
