Figure 2: IHC showing the expression of integrin β1 and NICD on day0 (normal skin), day 4 (post-injury) and day 22 (the time point when oxygen tension back to normal) at the wound site. | Scientific Reports

Figure 2: IHC showing the expression of integrin β1 and NICD on day0 (normal skin), day 4 (post-injury) and day 22 (the time point when oxygen tension back to normal) at the wound site.

From: Notch1 Signaling Contributes to Hypoxia-induced High Expression of Integrin β1 in Keratinocyte Migration

Figure 2

Images of skin tissue sections that were immunohistochemically stained for NICD. The tissues showed significantly lower levels of NICD (C) at the wound edge than those were observed (A) in normal skin. Images of skin tissue sections stained for integrin β1 showing that higher levels of integrin β1 were expressed (D) at the wound edge than (B) in normal skin. Both NICD and integrin β1 levels came back to normal (E,F) on day22. Scale bar indicates 200 μm. (G,H) The corresponding Integral optical density (IOD) for NICD and integrin β1 was measured at the indicated times. Values represent the means ± S.D. (n = 3~6 sections per group). *P < 0.05 versus normal skin (day 0 group); #P < 0.05 versus wound edge (day 4 group).

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