Figure 4: The effect of regulating Notch1 signaling on the expression of integrin β1.

(A) Western blot and a quantitative analysis showed that exposure to DAPT (10 μM or 20 μM, 24 h) decreased the expression of NICD and increased the expression of integrin β1. (B) Western blot and a quantitative analysis showed that the expression of NICD was down-regulated and the expression of integrin β1 was up-regulated after interference of Notch1 compared with a negative control (siRNA con). SiRNA interference lasted 6 h, and then cells were cultured for 24 h before harvest. (C) Western blot and a quantitative analysis showed that Jagged-1 (1 μg/ml, 24 h) or Dll4 (1 μg/ml, 24 h) effectively activated Notch1 signaling and reduced the expression of integrin β1 in normoxia. (D) Western blot and a quantitative analysis showed that Jagged-1 (1 μg/ml, 24 h) or Dll4 (1 μg/ml, 24 h) effectively reduced the hypoxia-induced increase in the expression of integrin β1. Values represent the means ± S.D. of three independent experiments. Values were normalized after comparison with control group or siRNA con group. *P < 0.05 versus corresponding column in the control group or siRNA con group; and ^#P < 0.05 versus corresponding column in the Hypoxia group.