Figure 5: Effects of Nr1d1-knockdown on neuronal migration during corticogenesis. | Scientific Reports

Figure 5: Effects of Nr1d1-knockdown on neuronal migration during corticogenesis.

From: Role of a circadian-relevant gene NR1D1 in brain development: possible involvement in the pathophysiology of autism spectrum disorders

Figure 5

(A) pCAG-GFP was coelectroporated with control pSUPER vector (a), pSUPER-mNR1D1 #1 (b) or #3 (c) into cerebral cortices at E14.5. For the rescue experiments, pCAG-GFP was coelectroporated with pSUPER-mNR1D1#1 together with pCAG-Myc-Nr1d1-R (d) or -Nr1d1-R500H (e). Coronal sections were prepared at P2. Nuclei were stained with DAPI (blue). Dotted lines represent the pial and ventricular surfaces. Bar, 100 μm. (B) Quantification of the distribution of GFP-positive neurons in distinct regions of the cerebral cortex for each condition shown in (A). Error bars indicate SD (n = 3); ***p = 0.0005 (bin1; control vs RNAi#1), ***p = 0.0002 (bin1; control vs RNAi#3), ***p = 0.0002 (bin3; control vs RNAi#1), **p = 0.0046 (bin3; control vs RNAi#3), ***p < 0.0001 (bin4; control vs RNAi#1), ***p < 0.0001 (bin4; control vs RNAi#3), *p = 0.0128 (bin5; control vs RNAi#3), ###p = 0.001 (bin1; RNAi#1 vs RNAi#1 + Nr1d1-R), ###p < 0.0001 (bin3; RNAi#1 vs RNAi#1 + Nr1d1-R), ###p < 0.0001 (bin4; RNAi#1 vs RNAi#1 + Nr1d1-R), $$$p = 0.0002 (bin1; RNAi#1 + Nr1d1-R vs RNAi#1 + Nr1d1-R500H), $$$p = 0.0006 (bin3; RNAi#1 + Nr1d1-R vs RNAi#1 + Nr1d1-R500H), $$$p < 0.0001 (bin4; RNAi#1 + Nr1d1-R vs RNAi#1 + Nr1d1-R500H) by Fisher’s LSD. (C) Representative images of control and Nr1d1-deficient neurons migrating in lower CP. Bar, 5 μm. (D) pCAG-GFP was coelectroporated with control pSUPER vector, pSUPER-mNR1D1#1 or #3 into cerebral cortices at E14.5. Coronal sections were prepared at P7. Quantification of the distribution of GFP-positive neurons in distinct regions was performed as in (B). Error bars indicate SD (n = 3).

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