Figure 9: Role of Nr1d1 in the dendrite growth in cortical neurons in vivo. | Scientific Reports

Figure 9: Role of Nr1d1 in the dendrite growth in cortical neurons in vivo.

From: Role of a circadian-relevant gene NR1D1 in brain development: possible involvement in the pathophysiology of autism spectrum disorders

Figure 9

(A) pCAG-loxP-RFP was electroporated for sparse expression with pCAG-M-Cre together with control pSUPER vector, pSUPER-mNR1D1#1 or pSUPER-mNR1D1#1 + pCAG-Myc-Nr1d1-R into cerebral cortices at E14.5. Analyses were carried out in cortical slices at P30. Representative average Z-stack projection images of RFP fluorescence of cortical neurons in upper CP are shown. Bar, 50 μm. (B,C) Number of dendritic branch point (B) or total dendritic length (C) was calculated on neurons observed in (A). Three brains were analyzed for each experiment; Control, n = 12 neurons; pSUPER-mNR1D1#1, n = 22; pSUPER-mNR1D1#1 + pCAG-Myc-Nr1d1-R, n = 20. Error bars indicate SD; ***p < 0.001 (control vs RNAi#1), *p < 0.01 (control vs RNAi#1), ##p < 0.01 (RNAi#1 vs RNAi#1 + Nr1d1-R), #p < 0.05 (RNAi#1 vs RNAi#1 + Nr1d1-R) by Fisher’s LSD.

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