Figure 2: The design and identification of the recombinant virus. | Scientific Reports

Figure 2: The design and identification of the recombinant virus.

From: Construction and immunogenicity of a recombinant swinepox virus expressing a multi-epitope peptide for porcine reproductive and respiratory syndrome virus

Figure 2

(A) The mP2 was constructed from three repeats of the P2 mimotope, with three glycine residues and a serine residue (GGGS) as a spacer peptide. (B) The construction of the pUSG11/P28-mp2 transfer vector. LF and RF respectively, indicate the SPV left and right flanking sequences, respectively. P11 and P28 are the vaccinia virus (VV) promoters. The GFP reporter gene is also included in the plasmid. (C) The recombinant virus rSPV-mp2 was constructed from the wtSPV homologous recombinant pUSG11/P28-mp2. (D) Western blot analysis of mP2 expression in PK-15 cells (uncropped): Lane 1: PK-15 cells infected with rSPV-mp2; Lane 2: PK-15 cells infected with wtSPV. (E,F) Indirect immunofluorescence assay of the rSPV-mp2. (E) Red fluorescence was observed in rSPV-mp2 infected PK15 cells, in which the fluorescence was localized to the cytoplasm. (F) No fluorescence was observed in PK15 cells infected with wtSPV.

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