Figure 3

(A) Percentage of cells showing lignification in pearl millet seedlings at different time intervals with (inoculated) or without (uninoculated) Sclerospora graminicola inoculation. Resistant – Seedlings of downy mildew resistant cultivar, Chitosan-Seedlings treated with Chitosan, T. hamatum UOM 13-Seedlings treated with the endophyte T. hamatum UOM 13, Control: Seedlings of downy mildew susceptible cultivar. Vertical bars indicate standard error. Results are average of three independent experiments with four replicates of 25 seedlings each. (B) Light microscopic (bright field) pictures showing the deposition of lignin in epidermal peelings from the coleoptile region pearl millet seedlings 24 h after inoculation with Sclerospora graminicola. Lignification detected by phloroglucinol-HCL staining method.