Figure 8: qRT-PCR determined relative expression of genes of various defense enzymes in two-day-old pearl millet seedlings with (I) or without (U) Sclerospora graminicola inoculation harvested 0, 3, 6, 9, 12, 24, 48, and 72 h. | Scientific Reports

Figure 8: qRT-PCR determined relative expression of genes of various defense enzymes in two-day-old pearl millet seedlings with (I) or without (U) Sclerospora graminicola inoculation harvested 0, 3, 6, 9, 12, 24, 48, and 72 h.

From: Elicitation of resistance and associated defense responses in Trichoderma hamatum induced protection against pearl millet downy mildew pathogen

Figure 8

Resistant – Seedlings of downy mildew resistant cultivar, Chitosan-Seedlings treated with Chitosan, T. hamatum UOM 13-Seedlings treated with the endophyte T. hamatum UOM 13, Control: Seedlings of downy mildew susceptible cultivar. (A) Phenylalanine ammonia lyase (B) Peroxidase (C) β-1,3 Glucanase (D) Polyphenol oxidase (E) PR-1 (F) PR-5 and (G) hydroxyproline-rich glycoprotein. Expression levels were measured by qPCR and normalized to the constitutive PP2A gene. Values are means of a single experiment carried out in triplicate. The bars indicate ± SE and the data were analyzed by one-way ANOVA followed by Tukey’s test and p-value < or = 0.05 was significant compared with control and <0.01 significant with treated control.

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