Table 2 Primer sequences used for qRT-PCR amplification.
Sl. No. | Target gene amplified | Forward primer sequence (5’ to 3’) | Reverse primer sequence (5’ to 3’) |
|---|---|---|---|
1. | Phenyl alanine ammonia lyase | ATGGAGTGCGAGAACGGCC | CTGCGCGATGCTGAGGCT |
2. | Peroxidase | CCCCAGAAGCACATTTGTGA | CATGGCTGCGGGCGGAG |
3. | β -1,3-Glucanase | ATGGCGAGGCAGGGTGTCATC | GGATTGGACTCCTGTTTA |
4. | Polyphenol oxidase | AGTCGAGGTTTGGCCACCAT | CCACCTGATGCGCTCGATG |
5. | PR1 | TGGACGTGCCGCTGCCG | GAACTGCGCCGCCACACG |
6. | PR-5 | GCGTCCTCGGTCCTCCTG | CACACGCGGCCGGAGCTG |
7. | Hydorxy rich glyco proteins | GCCTAAGCCGAAGCCACCAA | GCGTGTAGGTCGGAGGAGTT |
8. | DAHP Synthase | GGC TCA ATT TCA GGT ACC | GTT AGA GTC GGT AAG TAA |
9. | Shikimate Kinase | CTC ACC TAC CTC TCT CTC A | AAG GCT CTG CGA AAC TCT |
10. | Chorismate Synthase | ATC TTC CAA TCT TCA TATA | TCC TAG GTG TGG TAA TTC |
11. | Chorismate Mutase | CTT CAA TCT AAG GTT GGT AG | CTG AAT ATC ACA GGA AGC AG |
12. | IC Synthase | CAG GTT GAG TTT GAT GAG CT | CTT GAT AAG CAT CGG GTT |
Reference housekeeping gene | |||
1. | Protein phosphatase 2A | TGAGAGCAGACAAATCACTCAA | AAGAGCTGTGAGAGGCAAATAA |
