Figure 2: Inhibition of CYP1 activity regulates AHR pathway related targets in human PBMCs.
From: Cytochrome P450s in human immune cells regulate IL-22 and c-Kit via an AHR feedback loop
Human PBMCs were activated with anti-CD3/28 antibodies and treated with the AHR agonist FICZ (0.5 nM), the CYP1 inhibitor 1-PP (1 μM) and the AHR antagonist CH-223191 (3 μM) for 5 days. Inhibition of CYP1 activity increased RNA levels of (a) CYP1A1, (b) CYP1B1, (d) NQO1, and the (e) KIT genes, and slightly down-regulated (c) AHR transcription levels. CYP1 inhibition also increased IL-22, but decreased IL-17 on RNA (f and g), and on protein (h and i) level. Supplementation with the AHR antagonist inverted CYP1-induced effects. RNA expression levels were analyzed by qRT-PCR. Expression values (Ct values) were normalized by the housekeeping gene EF1A and related to the target gene expression in the medium control (ΔΔCt). Data were expressed as 2−(ΔΔCt) indicating relative fold regulation. Boxplots show medians, interquartile ranges and ranges of seven different subjects (*p < 0.05 by Wilcoxon signed-rank test).
