Figure 5: Inhibition of CYP1 activity amplifies c-Kit and IL-22 in purified human CD3+ T cells.
From: Cytochrome P450s in human immune cells regulate IL-22 and c-Kit via an AHR feedback loop
Human purified and activated CD3+ T cells were treated with increasing concentrations of the AHR agonist FICZ (1 pM–10 nM) alone, or in the presence of the CYP1 inhibitor 1-PP (1 μM) and the AHR antagonist CH-223191 (3 μM) for 4 days. (a and b) Increasing FICZ concentrations induced IL-22 and c-Kit. Data were indicated after subtraction of the DMSO control. (c) Induction of c-Kit+ T cells. Regulation of CD3+c-Kit+ T cells by FICZ alone (white circles), by FICZ and 1-PP (grey squares), or by FICZ, 1-PP and CH-223191 (black triangle). Means ± s.e.m. of six different subjects are shown. (d and e) Regulation of IL-22 and c-Kit in CD3+ T cells by FICZ (0.1 nM), 1-PP (1 μM) and CH-223191 (3 μM). Boxplots indicate data of six different subjects with medians, interquartile ranges and ranges (*p < 0.05, by Wilcoxon signed-rank test).
