Figure 7: HMGA1 knockdown influences EGFR pathways.

(a) Phopsho-kinase profiles in PC9/gef shLacZ and PC9/gef shC1-2 (HMGA1) obtained by human phospho-kinase array (R&D Systems, ARY003). Each membrane contains kinase specific (for example, 1−21) and positive control (P) antibodies spotted in duplicate. (b) Relative phosphorylation level was quantified by normalizing the pixel density of each positive control and shown in percentage. (c) Model of the mechanism of HMGA1-knockdown to recover sensitivity to gefitinib in resistant NSCLC.