Figure 2: Identification of S-layer proteins of L. salivarius REN. | Scientific Reports

Figure 2: Identification of S-layer proteins of L. salivarius REN.

From: The Adhesion of Lactobacillus salivarius REN to a Human Intestinal Epithelial Cell Line Requires S-layer Proteins

Figure 2

(A) SDS-PAGE of cell surface proteins extracted from L. salivarius REN. (B) Gene organization of the region surrounding the cbpA and nam-amidase locus. Arrows indicate the locations of primers used for PCR analysis. The red shaded regions represented the deletion target. The green and blue shaded regions represented the upstream and downstream fragments in target genes. The chromosomal map is not drawn to scale. (C) Schematic overview of the construction of Δ cbpA and Δ nam-amidase mutants. (D) PCR identification of double crossover recombinant. Lane M: DNA marker DL 2000, lane 1: PCR amplification of cbpA in Δ cbpA mutants, lane 2: PCR amplification of cbpA in wild type strain, lane 3: PCR amplification of nam-amidase in wild type strain, lane 4: PCR amplification of nam-amidase in Δ nam-amidase mutants. (E) Adhesion of L. salivarius REN, Δ cbpA and Δ nam-amidase mutants to the HT-29 cells. Data were analyzed by the paired Student’s t test (*P < 0.05; **P < 0.001). Bars represented mean values of three independent experiments.

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