Figure 3: Total pTyr level in RIN-5AH beta cells, after treatment with various quinoline derivatives. | Scientific Reports

Figure 3: Total pTyr level in RIN-5AH beta cells, after treatment with various quinoline derivatives.

From: Novel members of quinoline compound family enhance insulin secretion in RIN-5AH beta cells and in rat pancreatic islet microtissue

Figure 3

(a) Gated cells for evaluation. (b) Representative graphs of fluorescence intensities measured in FL2-H. Fluorescence which is proportional to pTyr level is significantly reduced after treatment with compound 15, compound 12 and bosutinib, while compound 8 and compound 9 didn’t affect tyrosine phosphorylation. (c) Quantitative evaluation of total pTyr levels in RIN-5AH cells after treatment with quinoline compounds and bosutinib (BOS) at 5 μM for 2 h. Grey bars indicate treatments with 2-ciano-quinoline derivatives, black bars stand for 3-ciano-quinolne derivatives. Data is normalized to samples incubated with isotype control Ab and represented in % to DMSO (DMSO set to 100%). (n = 3; SEM; ANOVA/Tukey).

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