Figure 2: Tivozanib induces G2/M cell cycle arrest. | Scientific Reports

Figure 2: Tivozanib induces G2/M cell cycle arrest.

From: Blockade of vascular endothelial growth factor receptors by tivozanib has potential anti-tumour effects on human glioblastoma cells

Figure 2

(A) Following treatment with tivozanib for 48 h, the cell pellets were fixed and incubated with PI to analyse the cell cycle distribution on a flow cytometer. The graphs are representative of three independent experiments with similar results. (B) The cells were treated with tivozanib for 48 h then total RNA was harvested for qRT-PCR analysis. (C) Protein lysates from tivozanib-treated cells were subjected to Western blotting and probed with the indicated antibodies. β-actin was used as the loading control. The blots are representative of three independent experiments with similar outcomes. Data are given as mean ± SD. Statistically significant values of *p < 0.05, **p < 0.01, and ***p < 0.001 were determined compared with the control. AURK, aurora kinase; FOXM1, forkhead box M1; CCNB, cyclin B; NEK2, NIMA related kinase 2; CENPF, centromere protein F; IL, interleukin; CDKN1A, cyclin-dependent kinase inhibitor 1 A; CDK, cyclin-dependent kinase; CCNA2, cyclin A2; GADD45A, growth arrest and DNA damage inducible alpha; CHEK, checkpoint kinase; CDC25, cell division cycle 25; MYT1, myelin transcription factor 1; SFN, stratifin.

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