Figure 1: R834C-WRN is expressed in homozygous cells. | Scientific Reports

Figure 1: R834C-WRN is expressed in homozygous cells.

From: Homozygosity for the WRN Helicase-Inactivating Variant, R834C, does not confer a Werner syndrome clinical phenotype

Figure 1

Lysates (100 μg total protein) from control and TT homozygous cells from four pedigrees, F14, F19, F26, and F33, were electrophoresed through a 4–12% polyacrylamide gel. The proteins were blotted to a PVDF membrane, and probed with anti-WRN antibody and, simultaneously with anti-nucleolin antibody, to control for loading differences. The membrane was incubated with AlexaFluor 647-labeled anti-mouse antibody, and protein bands were visualized on an AlphaInnotech FluorChemQ Imaging Station. Band intensities were quantified using ImageJ software. CC: wild type controls; TT: R834C-WRN expressing cells; M: Molecular weight standards; numbers on the left indicate protein sizes in kDa.

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